Figure 4.

Knockdown of FGFR3 inhibits the expression of TGF‐β1‐induced fibrosis‐associated molecules. CCD18‐Lu cells were treated with 10 μM of AZD4547 for 2 h, following treatment with TGF‐β1 (1 ng/mL) for 5 h. Total protein was isolated and analysed by Western blotting for the indicated proteins. A, Signalling molecules. C, Fibrotic markers. CCD18‐Lu cells were transfected with scramble RNA or siFGFR3, and then incubated for 24 h. TGF‐β1 (1 ng/mL) was added to the cells, and the cells were incubated for an additional 5 h. Total protein was isolated and analysed by Western blotting for the indicated proteins. B, Signalling molecules. D, Fibrotic markers