Figure 4.

MiR‐1275 attenuated radioresistance of EC cells through targeting WNT1‐activated Wnt/β‐catenin pathway. A, Transfection efficiency of si‐NC or si‐WNT1 in KYSE‐150 cells and that of pcDNA3.1 or pcDNA3.1/WNT1 inKYSE‐150R cells transfected with was tested via qRT‐PCR. B, Protein levels of WNT1 in EC cells at different groups were determined by Western blot. C, TOP/FOP flash assay for the detection of Wnt/β‐catenin activity in various groups. D, The survival fraction of KYSE‐150 and KYSE‐150R cells under irradiation was assessed by colony formation assay. E, The cell viability of above cells was examined by MTT assay. *P < .05, **P < .01