Figure 5.

MiR‐1275 inhibited EMT in EC cells by abating WNT1 expression. KYSE‐150 cells were transfected with NC inhibitor, miR‐1275 inhibitor or miR‐1275 inhibitor together with si‐WNT1 and KYSE‐150R cells were transfected with NC mimic, miR‐1275 mimic or miR‐1275 mimic together with pcDNA3.1/WNT1. A, B, Wound‐healing assay was performed to evaluate cell migration in above KYSE‐150 cells and KYSE‐150R cells under different conditions. C, Transwell migration assay was also conducted in the above EC cells to assess cell migration. D, The level of EMT‐related proteins in indicated cells was detected by Western blot. *P < .05, **P < .01