Figure 3.

SPRY4‐IT1 affects cell stemness maintenance of MCF‐7 and T47D CSCs. A, Expression of stemness markers (SOX2, OCT4 and Nanog) was detected by qRT‐PCR in MCF‐7 and T47D CSCs transfected with two sh‐SPRY4‐IT1. B, Left: Expression of stemness markers (Nanog, SOX2 and OCT4) was detected in sh‐SPRY4‐IT1 MCF‐7 and T47D CSCs by western blotting. Right: Densitometric analysis of protein expression. C, Sphere formation assays showed the mammosphere‐forming ability of MCF‐7 and T47D CSCs transfected with sh‐SPRY4‐IT1. Original magnification, ×40. Scale bars, 100 μm. D, Flow cytometry indicated the CD44+/CD24 − percentage in MCF‐7 and T47D CSCs transfected with sh‐SPRY4‐IT1. Data are presented as the mean ± SD of three independent experiments performed in triplicate. *P < .05, **P < .01, ***P < .001, ****P < .0001