Figure 3.

Validation of expression of several differentially expressed exosomal proteins. A, The representative image of expression of up‐regulated exosomal proteins S100‐A9, peroxiredoxin 6, APMAP and angiotensinogen by Western blot in exosomes from normal and PCOS follicular fluid (n = 6 per group). B, Concentration of S100‐A9 in follicular fluid supernatant (100 µL) and exosome lysis solution (exosomes were isolated from 100 µL FF, repeated freeze‐thaw cycles three times to gain lysis solution) measured by ELISA (n = 18 per group), *P < .05. All data were presented as means ± SEM. Exo‐lysis, exosome lysis solution; FF, follicular fluid; NC, normal control