Table 1.

Primers for real‐time RT‐PCR

Name	Sequence（5′‐3′）	Product size
α‐SMA	F: GACAATGGCTCTGGGCTCTGTAA	147 bp
	R: CTGTGCTTCGTCACCCACGTA
Col1A1	F: CCCGGGTTTCAGAGACAACTTC	148 bp
	R: TCCACATGCTTTATTCCAGCAATC
GAPDH	F: GGATTTGGTCGTATTGGG	205 bp
	R: GGAAGATGGTGATGGGATT
ZEB1	F: AACGCTTTTCCCATTCTGGC	167 bp
	R: TTGCCGTATCTGTGGTCGTG
Col3A1	F: AATCAGGTAGACCCGGACGA	284 bp
	R: TCGAGCACCGTCATTACCC
miR‐590‐3p	F: ACACTCCAGCTGGGGAGCTTATTCATAA	52 bp
	R: CAGTGCAGGGTCCGAGGTAT
U6	F: CTGGTAGGGTGCTCGCTTCGGCAG	150 bp
	R: CAACTGGTGTCGTGGAGTCGGC
ZEB1‐3’UTR	F: CCTCGAGTGTATGTCTTCAAACCTGGCAGT*	191 bp
	R: GCGTCGACTGTTCTACAGTCCAAGGCAAGT*
ZEB1‐3’UTR‐MUT	MR: TCTTATCAACTTTCCACGCGCGCATACTAAAATATAT*	126 bp
	MF: ATATATTTTAGTATGCGCGCGTGGAAAGTTGATAAGA*
Si‐ZEB1	ATGACATGAAGCTTTGTATCTCC	23
Si‐ZEB2	CTGAAACACTGGGACATTTCATC	23
Si‐ZEB3	AGGGACTAACAATGTTAATCTGA	23

^*The bold part is base protection. The italics are mutation of part of miR‐590‐3p binding site sequence.