Figure 2.

Knockdown of TUG1 attenuates the OGD/R injury by inhibiting the expression of AQP4. A, AQP4 protein levels in MA‐C cells transfected with short interfering RNAs siTUG1‐1, siTUG1‐2 and siTUG1‐3 as assessed using by Western blotting assays. B, LDH leakage (left) and cell viability were measured using LDH and CCK8 assays, respectively. MA‐C cells were transfected with siTUG1‐1, siTUG1‐2 and siTUG1‐3 in 6‐h OGD/24‐h reoxygenation treatments. C, Flow cytometry assays were performed to show the cell apoptosis after cotransfection with AQP4 siRNA and siTUG1‐1, siTUG1‐2 and siTUG1‐3 in 6‐h OGD/24‐h reoxygenation treatments. AQP4, aquaporin 4; OGD/R, oxygen‐glucose deprivation and reperfusion; siRNA, short interfering RNA; TUG1, taurine‐up‐regulated gene 1