Figure 1.

BMX regulates VEGFR2 expression but not VEGFR2 protein stability in ECs. HUVECs were transfected with human BMX siRNA or control siRNA (20 nmol/L) for 48 h. A, Total VEGFR2 and BMX proteins were determined by Western blotting with specific antibodies. β‐Actin was used as a loading control. B‐D, BMX siRNA on VEGFR2 stability. siRNA‐transfected HUVECs were incubated with cycloheximide (CHX, 10 μg/mL) for the indicated time‐points. Total VEGFR2 and BMX proteins were determined by Western blotting with specific antibodies. β‐Actin was used as a loading control (B). The protein bands in B were quantified by densitometry, and the relative VEGFR2 levels were presented by setting untreated control siRNA as 1.0 (C). Normalized VEGFR2 expression shows its decay rate (D). E‐F, BMX siRNA on VEGFR2 stability in the presence of VEGF‐A. siRNA‐transfected HUVECs were incubated with cycloheximide (CHX, 10 μg/mL) in the presence of VEGF‐A (50 ng/mL) for the indicated time‐points. VEGFR2 protein detection (E) and normalization (F) as described in B‐D. The data are means ± SEM from three independent experiments