Figure 2.

BMX regulates VEGFR2 expression at the transcriptional level in ECs. HUVECs were transfected with human BMX siRNA or control siRNA (20 nmol/L) for 48 h. A, siRNA‐transfected HUVECs were incubated with actinomycin D (10 μg/mL) for the indicated time‐points. VEGFR2 mRNA was assessed at the indicated time‐points by qRT‐PCR with normalization to β‐actin mRNA. B, The % decrease of VEGFR2 mRNA due to actinomycin D was quantified normalized to Ctrl siRNA at 0 min, using the time 0 of each respective group as the reference point. C, Normalized VEGFR2 mRNA levels. The data are means ± SEM from three independent experiments. **, P < .01