Figure 3.

PVT1 promoted the apoptosis and repressed the proliferation of HLE B‐3 cells in HG. A, The short hairpin RNAs (shRNAs) targeting the PVT1 were chemically synthesized to silence its expression. B, CCK‐8 proliferative ability analysis showed the proliferation of HLE B‐3 cells treated with normal glucose (NG), high glucose (HG) and PVT1 silencing transfection. C, D, EdU assay illustrated the proliferation of HLE B‐3 cells treated with normal glucose (NG), high glucose (HG) and PVT1 silencing transfection. E, F, Flow cytometry analysis unveiled the apoptotic rate of HLE B‐3 cells treated with normal glucose (NG), high glucose (HG) and PVT1 silencing transfection. Data were presented as mean ± SD. *P < .1, **P < .01