Figure 5.

MMP2 acted as the target of miR‐214‐3p. A, The potential target of miR‐214‐3p was predicted using the bioinformatics tools. B, Luciferase reporter gene reporter assay illustrated the interaction within MMP2 and miR‐214‐3p. WT presents the wild‐type plasmids, and the Mut presents the mutant plasmids. C, RT‐qPCR unveiled the MMP2 mRNA level in HLE B‐3 cells transfected with miR‐214‐3p inhibitor or miR‐214‐3p mimics and their controls. D, Western blot analysis indicated the MMP2 protein level in HLE B‐3 cells transfected with miR‐214‐3p inhibitor or miR‐214‐3p mimics and their controls. E, Western blot analysis showed the MMP2 protein levels with PVT1 silencing shRNA transfection. Data were presented as mean ± SD. **P < .01