Figure 6.

GSS ameliorates sepsis‐induced lung vascular endothelial apoptosis though the BCL‐2 protein. Mouse lungs were harvested 24 h after CLP application. A, The expression of cleaved PARP and total PARP in lung tissue samples was evaluated by Western blot. B, The Western blot results are presented as a histogram showing the band intensity values. C, Expression of BCL‐2 in lung tissue samples was evaluated by Western blot. D, The Western blot results are presented as a histogram showing the band intensity values. E, The lung vascular endothelium and apoptotic cells were stained for CD31 (red) and TUNEL (green), and the results were visualized by immunofluorescence microscopy. The white arrow in the control group and GSS group represents the complete vascular barrier. The arrow in the CLP group represents vascular barrier disruption and endothelial apoptosis. The arrow in the CLP + GSS group shows that vascular barrier disruption is reversed. F, The expression of Myd88 in lung tissue samples was evaluated by Western blot. G, The Western blot results are presented as a histogram showing the band intensity values. H, The expression of P‐P65 and P65 in lung tissue samples were evaluated by Western blot. I, The Western blot results are presented as a histogram showing the band intensity values. J, The expression of TLR4 in lung tissue samples was evaluated by Western blot. K, The Western blot results are presented as a histogram showing the band intensity values. *P < .05 vs negative control. ^# P < .05 vs the corresponding LPS treatment group