Figure 7.

GSS inhibits sepsis‐induced acute lung injury and vascular barrier dysfunction in mice. Mouse lungs were harvested 24 h after CLP application. A, Sepsis in C57BL/6J mice was induced by the CLP procedure after pre‐treatment with GSS or special inhibitor for 2 h, and the histological analysis of lung tissue was analysed by haematoxylin and eosin staining. B, Evans blue dye was injected 2 h before termination of the experiment, and the Evans blue extravasation was photographed in different groups. C, The quantitation of Evans blue extravasation was performed by spectrophotometric analysis of Evans blue extracted from the lung tissue samples. D, Wet/dry ratio of lungs was represented as a histogram according to the collected data. E, The PaO2/FiO2 ratio was detected after CLP in mice with and without GSS or inhibitor. F, The survival rate was detected after CLP in mice with and without GSS or special inhibitor. *P < .05 vs negative control. ^# P < .05 vs the corresponding LPS treatment group