Figure 2.
Hypoxia induces 14‐3‐3ζ expression and promotes autophagy in HCC‐LM3 and CSQT‐2 cells. HCC‐LM3 and CSQT‐2 cells were cultured in 2% oxygen (Hypoxia) or 95% air (Normoxia) for 6, 12 or 24 h. (A,B) The protein levels of 14‐3‐3ζ and beclin 1, phospho‐beclin 1S295, LC3 and p62 were determined with Western blot analysis. Cancer cells were cultured under hypoxia for 12 h, and (C,E) immunofluorescence was performed to probe 14‐3‐3ζ and beclin 1 expression. Green fluorescence marked 14‐3‐3ζ, whereas red fluorescence marked beclin 1. (D,F) 14‐3‐3ζ‐ and beclin 1‐positive cells were counted and shown as a percentage. A *P value <.05 was considered significant