Figure 6.

miR‐485‐3p reduced proliferation and induced differentiation of NSCs via targeting TRIP6 expression. A, Ectopic expression of TRIP6 increased miR‐485‐3p‐overexpressing NSCs proliferation with using MTT assay. B, The expression of nestin was determined by using qRT‐PCR assay. C, The expression of ki‐67 was measured by using qRT‐PCR assay. D, The expression of cyclin D1 was measured by using qRT‐PCR assay. E, Restoration expression of TRIP6 over‐turned the function effect of miR‐485‐3p on NSCs differentiation. F, As measured by Tuj1 immunofluorescence analysis, results indicated that restoration expression of TRIP6 decreased the Tuj1 expression in the miR‐485‐3p‐overexpressing NSCs. ^* P < .05 and ^** P < .01