Figure 5.

Loss of CCM protein family members increases VEGFR2 tyrosine phosphorylation. A, Tyrosine phosphorylation of VEGFR2 in HPAEC transfected with negative control (NC), anti‐KRIT1, anti‐CCM2 and anti‐CCM3 siRNA. IB, immunoblot; IP, immunoprecipitation. Blots are representative, n = 3. B, Densitometric quantification of blots in (A). Data shown are P‐Tyr normalized to total VEGFR2, ± SEM, P = .0223 by ANOVA, *P < .05 by post hoc testing vs NC transfected cells. C, VEGF protein (pg/mL) in conditioned media harvested from cells expressing anti‐KRIT1, anti‐CCM2 and anti‐CCM3 siRNA P = .032 by ANOVA, *P < .05 by post hoc testing vs NC transfected cells