Figure 3.

GTIIC reporter activity and the protein levels of CDK7, YAP and YAP downstream genes in MPM cell lines after knockdown of CDK7. GTIIC reporter activity was measured in three cell lines after CDK7 inhibition by siRNA1, 2. A, 211H cells. B, H2052 cells. C, H290 cells. D, The protein expression of CDK7, YAP, pYAP and YAP downstream genes (Cyr61 and CTGF) was detected by Western blot after CDK7 inhibition by siRNA1, 2. Expression was compared with their respective control cells treated with non‐targeting siRNA (vs siRNA‐ctrl group, *P < .05, **P ≤ .01, ***P ≤ .001, One‐way ANOVA, Scheffe multiple comparisons). GAPDH was used as a loading control. Band intensity was analysed with ImageJ software and normalized to the intensity of the GAPDH band