Figure 6.

Expression of CDK7 and YAP after CDK7 forced restoration in siRNA‐CDK7 silenced MPM cells and Co‐IP experiment. A, B, Western blot analysis of YAP, CDK7 after CDK7 silencing by siRNA and/or forced restoration of the CDK7 gene in 211H and H2052 cells. GAPDH was used as a loading control. Band intensity was analysed with ImageJ software and normalized to the intensity of the GAPDH band. C, Co‐Immunoprecipitation in 211H cells. Protein expression (YAP, CDK7 and Tead4) from co‐IP was tested by Western blot. The studies using a YAP‐specific monoclonal antibody resulted in CDK7 protein capture. D, Schematic of the potential mechanism through which CDK7 increases YAP protein stability. CDK7 inhibition promotes YAP protein degradation