Figure 4.

TLR4 is a molecular target of miR‐148a. A, The schematic graph displayed pairing relationship between miR‐148a and TLR4 mRNA 3′‐UTR. The dual‐luciferase reporter assay was carried out in HEK293T cells. Cells were cotransfected with the wild‐ or mutant‐type reporter vectors, as well as miR‐148a agomiR or the negative control (NC agomiR). The ratio of Renilla activity/Firefly activity represents luciferase activity. B, BEND cells were transfected with miR‐148a agomiR, miR‐148a antagomiR, or the corresponding negative controls (NC agomiR, NC antagomiR), and then, the TLR4 protein levels were measured by Western blotting. C, Immunofluorescence staining of TLR4 in BEND cells transfected with miR‐148a agomiR or miR‐148a antagomiR. Data are presented as the mean ± SEM. *P < .05; **P < .01