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Primer sequences for RT‐qPCR
| Gene | Sequence (5'‐3') |
|---|---|
| miR‐27a | F: TTCACAGTGGCTAAG |
| R: GTGCAGGGTCCGAGGT | |
| BTG2 | F: GCGCGGGCTCTTCCTCTTTG |
| R: AAGGAAGGCTGGAAGAGTGC | |
| β‐actin | F: TGGAGGGGCCGGACTCGTCA |
| R: CTTCCTTCCTGGGCATGGAG | |
| U6 | F: GCTTCGGCAGCACATATACTAAAAT |
| R: CGCTTCACGAATTTGCGTGTCAT |
Abbreviations: BTG2, B‐cell translocation gene 2; F, forward; miR‐27a, microRNA‐27a; R, reverse; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction.
