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. 2019 Nov 14;24(1):785–798. doi: 10.1111/jcmm.14790

Figure 4.

Figure 4

l‐tetrahydropalmatine (l‐THP) inhibits RANKL‐induced activation of NF‐κB and MAPK pathway. A, Induced RAW264.7 cells treated with l‐THP (19.00 μg/mL, 1h) were fixed with 40% formaldehyde, washed by Triton X‐100, followed by incubation with anti‐P65 antibody, goat antimouse IgG antibody and fluorescein‐conjugated streptavidin. B, Induced RAW264.7 cells treated with l‐THP (19.00 μg/mL) for 0, 15, 30 and 60 min. The levels of phosphorylated P65, P50 and IκB were evaluated by Western blotting analysis. C, Induced RAW264.7 cells treated with l‐THP (19.00 μg/mL) for 30 min, were then collected and resuspended with a 32P‐labelled DNA duplex and run in sodium dodecyl SDS‐PAGE system for EMSA analysis of the DNA binding activity of NF‐κB. D, Induced RAW 264.7 cells were treated with l‐THP (19.00 μg/mL) for 0, 15, 30 and 60 min, followed by Western blot analysis of the levels of phosphorylated ERK, JNK and P38. (*P < .05, **P < .01)