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. 2019 Dec 20;7:351. doi: 10.3389/fcell.2019.00351

FIGURE 6.

FIGURE 6

Beta cells differentiated from MSCs were transplanted below the renal capsule of STZ-treated diabetic mice. (A) Hematoxylin and eosin staining of a graft at day 40 after transplantation. Higher numbers of islet-like structures were observed in induced MSCs than in SB-431542-treated induced MSCs (red circles). The MSC-transplanted group did not exhibit any of these structures in the graft 18 days after transplantation. (B) Immunofluorescence images of a graft at day 40 after transplantation, stained for insulin and glucagon to confirm the presence of engrafted islets (scale bar = 50 μm). (C) Survival curves of diabetic mice following transplantation. Induced MSC-transplanted diabetic mice (n = 10) survived the longest with a survival probability reaching 60%, while the survival probability of SB-431542-treated induced MSC-transplanted diabetic mice (n = 10) only reached 20%, and that of the sham control and MSC-transplanted groups (n = 10) was 0%. (D) Blood glucose analysis of the various transplantation groups showed that the induced MSC-transplanted diabetic mice recovered to normal glucose levels (<13.9 mM) after 20 days. The other groups retained high levels of blood glucose (>13.9 mM) over the 40 days observation period.