Figure 4.

(A–D) Ca²⁺ dynamics induced by SOCE activation in neurons dissociated from larval brains expressing spastin^K467R, and relative control, as detected by fura-2. Were indicated, vinblastine was applied: (A,B) during development, larvae were chronically treated with 50 nM vinblastine or (C,D) after dissociation, neurons were acutely treated with 1 μM vinblastine. Cells were pre-treated with thapsigargin for 8 min to empty intracellular stores, then 2 mM CaCl₂ was added. Traces are presented as average R/R₀ values of n > 100 cells. (B,D) Histograms reporting the average area under curve (AUC) values of the corresponding traces. Data are presented as mean ± SEM of n > 100 cells. *p < 0.05; ***p < 0.001. (E) Flight ability, assayed by the cylinder drop test, of animals expressing spastin^K467R and relative control. Where indicated, animals were chronically exposed to vinblastine (50 nM). Data are presented as the percentage of flying individuals. Mean of n > 100 cells. ***p < 0.001.