Fig. 2.

Constrained tagging of Lifeact with cpGFP. (a) Names and sequences of cpGFPΔC mutants, linkers, and LifeactΔN mutants. (b) Schematic of constrained cpGFP-Lifeact. (c) Binding of cpGFP-Lifeact constructs to F-actin bundles. In the presence of 200 nM SiR-actin, HeLa cells expressing each construct were imaged by epifluorescence microscopy. (Scale bar: 10 μm) (d) Fluorescence anisotropy of cpGFP-Lifeact constructs on F-actin structures. HeLa cells expressing each construct were imaged by FPM in the presence of 200 nM SiR-actin. (Scale bar: 10 μm)