Figure 1.
Collagen I and III mRNA level are regulated in a sex-specific manner by E2 in human cardiac fibroblasts. (A) CF isolated from women and (B) men with AS after 12 h vehicle or 10−8 M E2-treatment. Collagen I and III mRNA level were quantified by qPCR and normalized to HPRT. Collagen I and III expression of E2-treated cells are given as fold induction of vehicle-treated cells. Mean ± SEM from 10 to 11 independent samples derived from four independently repeated experiments; **P ≤ 0.01, *P ≤ 0.05, comparison was performed using Mann–Whitney U-test.