Figure 3.
Collagen I and III mRNA level are regulated in a sex-specific manner by sex-dimorphic ERα- and ERβ-activation. (A) Female and (B) male rCF were analysed after 24 h vehicle, 10−8 M E2, 10−7 M ERα- and ERβ-agonist treatment. Collagen I and III mRNA levels were quantified by qPCR and normalized to HPRT. Collagen I and III expression of E2-, ERα-, and ERβ-agonist-treated cells are given as fold induction of vehicle-treated cells (indicated by the horizontal red line). Mean ± SEM from 9 to 12 independent samples derived from four independently repeated experiments; **P ≤ 0.01, *P ≤ 0.05, comparison between treated cells and respective control was performed using Mann–Whitney U-test.