Figure 5.
ER bind in a sex-specific manner within the collagen I and III promoter in rat cardiac fibroblasts in the presence of E2. (A–F) ERα and ERβ binding to the rat collagen I and III promoter upon 1 h 10−8 M E2-treatment in rCF was analysed using ChIP assays. (A) Upon E2-treatment, ERα and (B) ERβ bind to the collagen I promoter in female rCF (C), whereas only ERβ binds within the collagen I promoter in male rCF. (D) In female rCF, ERα and (E) ERβ bind within the collagen III promoter, and (F) in male rCF only ERβ binds collagen III promoter upon E2-treatment. Mean ± SEM from four to seven independently repeated experiments per sex. Comparisons between all four groups was performed by one-way ANOVA followed by Dunn's multiple comparison test; *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.