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. Author manuscript; available in PMC: 2020 Jul 18.
Published in final edited form as: Methods Enzymol. 2019 Jul 18;628:191–221. doi: 10.1016/bs.mie.2019.06.016

Figure 2.

Figure 2.

Schematics (CAD drawings) and photographic images of our inverted microscope-based chemical cytometry system. (A) Schematic of tilted top-down view of the automated xy-stage, fluorescence detection optical pathway, and cassette with cell array and fluidic compartments. (B) Photograph of tilted top-down view of the automated xy-stage. (C) CAD view of the side of the instrument. (D) Photograph of the entire assembly from the side. (E) CAD view from the front of the instrument assembly. (F) CAD top-down view of the microscope with the xy-stage, and CE employing fluorescence detection optical pathway removed. Legend: (i) xy-stage, (ii) capillary and fluorescence-detection system mount, (iii) xyz-micromanipulators, (iv) white LED, (v) custom fluidic cassette, (vi) CE optical path mount, (vii) capillary outlet holder & buffer vial, (viii) optical post, (ix) microscope revolving turret w/ objective, (x) Nd:YAG laser entry port, (xi) coarse/fine focus knobs, (xii) xy-stage mounts, (xiii) microscope filter-sets, (xiv) CCD camera.