Table 2.

Sequences of insertions encoding shRNA, targeting IRF3/7, TLR7/8, NOD2, RIG-I, MDA5, and PKR genes, and shRNA with scrambled sequence.

Designation	Sequence of shRNA encoding insertion
Scr	5′-p-GATCCCAAGTCTCGTATGTAGTGGTTCAAGAGACCACTACATACGAGACTTGTTTTTG-3′         3′-GGTTCAGAGCATACATCACCAAGTTCTCTGGTGATGTATGCTCTGAACAAAAACTTAA-p-5′
TLR7 (4,579–4,597)	5′-p-GATCCGGAGAAGAAACCAAAGTTTTTCAAGAGAAAACTTTGGTTTCTTCTCCTTTTTG-3′         3′-GCCTCTTCTTTGGTTTCAAAAAGTTCTCTTTTGAAACCAAAGAAGAGGAAAAACTTAA-p-5′
TLR8 (579–597)	5′-p-GATCCGGGCATTTCAAGACTTATATTCAAGAGATATAAGTCTTGAAATGCCCTTTTTG-3′         3′-GCCCGTAAAGTTCTGAATATAAGTTCTCTATATTCAGAACTTTACGGGAAAAACTTAA-p-5′
PKR (2,133–2,152)	5′-p-GATCCCGACCTAACACATCTGAAATTCAAGAGATTTCAGATGTGTTAGGTCGTTTTTG-3′         3′-GGCTGGATTGTGTAGACTTTAAGTTCTCTAAAGTCTACACAATCCAGCAAAAACTTAA-p-5′
MDA5 (3,253–3,272)	5′-p-GATCCGTGCCGACTATCAAATAAATTCAAGAGATTTATTTGATAGTCGGCACTTTTTG-3′         3′-GCACGGCTGATAGTTTATTTAAGTTCTCTAAATAAACTATCAGCCGTGAAAAACTTAA-p-5′
RIG I (1,103–1,122)	5′-p-GATCCAGACATGGGTATAGAGTTATTCAAGAGATAACTCTATACCCATGTCTTTTTTG-3′         3′-GTCTGTACCCATATCTCAATAAGTTCTCTATTGAGATATGGGTACAGAAAAAACTTAA-p-5′
NOD2 (926–944)	5′-p-GATCCCAAATGCCACCAGGAACTGTTCAAGAGACAGTTCCTGGTGGCATTTGTTTTTG-3′         3′-GGTTTACGGTGGTCCTTGACAAGTTCTCTGTCAAGGACCACCGTAAACAAAAACTTAA-p-5′
IRF3 (600–618)	5′-p-CCGGGATCTGATTACCTTCACGGAACTCGAGTTCCGTGAAGGTAATCAGATCTTTTTG-3′         3′-CTAGACTAATGGAAGTGCCTTGAGCTCAAGGCACTTCCATTAGTCTAGAAAAACTTAA-p-5′
IRF7 (1,298–1,317)	5′-p-GATCCGCACGTTCCTATACGGCCCTTCAAGAGAGGGCCGTATAGGAACGTGCTTTTTG-3′         3′-GCGTGCAAGGATATGCCGGGAAGTTCTCTCCCGGCATATCCTTGCACGAAAAACTTAA-p-5′