Table 3.
Relative mRNA level of potential isRNA sensors and signal transducers in KB-3-1 and A549 cells.
| Potential sensor of isRNA | Normalized mRNA levela × 103 | |
|---|---|---|
| KB-3-1 | A549 | |
| IRF3 | 8.5 ± 0.5 | 5.0 ± 0.9 |
| IRF7 | n. d.b | n. d. |
| MDA5 | 2.8 ± 0.4 | 0.8 ± 0.2 |
| NOD2 | n. d. | n. d. |
| PKR | 90.6 ± 5.6 | 13.6 ± 2.1 |
| RIG-I | 6.1 ± 0.3 | 2.1 ± 0.3 |
| TLR3 | n. d. | 0.5 ± 0.1 |
| TLR7 | n. d. | n. d. |
| TLR8 | n. d. | n. d. |
a
The mRNA level was measured by qRT-PCR and normalized to the GAPDH mRNA level. Experiments were performed in triplicate. The data represent means ± SD.
b
n.d.—relative mRNA level is below the reliable detection limit.