Table 4.
Inhibition of the expression of PRRs and transcription factors by shRNA in transduced KB-3-1 and A549 cell lines.
| Cell line | Target mRNA | Relative mRNA level, % of controla |
|---|---|---|
| KB-3-1-MDA5 | MDA5 | 45 ± 2 |
| KB-3-1-IRF3 | IRF3 | 32 ± 2 |
| KB-3-1-RIG-I | RIG-I | 36 ± 3 |
| KB-3-1-PKR | PKR | 14 ± 3 |
| A549-MDA5 | MDA5 | 7 ± 2 |
| A549-IRF3 | IRF3 | 22 ± 2 |
| A549-RIG-I | RIG-I | 6 ± 2 |
| A549-PKR | PKR | 18 ± 7 |
a
The relative mRNA level was measured by qRT-PCR. The relative level of mRNA of target genes in parent and scrambled-shRNA-expressing KB-3-1 and A549 cell sublines was set at 100%. GAPDH mRNA was used as internal standard. Experiments were performed in triplicate. The data represent mean ± SD.