Table 5.
The effect of PRRs gene silencing by shRNA on the antiproliferative activity of isRNA in KB-3-1 and A549 cell lines and sublines.
| Cell line | Silenced gene | Antiprolefirative effecta, % |
|---|---|---|
| KB-3-1 | * | 44 ± 11 |
| KB-3-1-Scr | * | 41 ± 11 |
| KB-3-1-MDA5 | MDA5 | 47 ± 7 |
| KB-3-1-IRF3 | IRF3 | 61 ± 5 |
| KB-3-1-RIG-I | RIG-I | 8 ± 8 |
| KB-3-1-PKR | PKR | 2 ± 4 |
| A549 | * | 35 ± 7 |
| A549-Scr | * | 50 ± 8 |
| A549-MDA5 | MDA5 | 68 ± 11 |
| A549-IRF3 | IRF3 | 63 ± 11 |
| A549-RIG-I | RIG-I | 9 ± 7 |
| A549-PKR | PKR | 8 ± 8 |
*
no genes were silenced.
a
Antiproliferative effect (%)—the decrease in the number of living cells 96 h after transfection with isRNA/2X3-DOPE compared to the mock-treated cells. The relative number of cells treated with 2X3-DOPE alone was set at 100%.