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. 2019 Dec 20;9:1450. doi: 10.3389/fonc.2019.01450

Figure 2.

Figure 2

Ectopic expression of SIX1 increased the proportion of cells in S phase and promoted proliferation, migration and invasion in BCPAP cell lines. Protein abundance of SIX1 in PTC cell lines by western blot analysis (A). Western blot analysis confirmed the efficiency of ectopic expressing SIX1 in BCPAP cells or silencing SIX1 in TPC-1 cells (B). Representative images of cell cycle plot (C) and quantitative results (n = 3) (D). Quantitative results of EdU staining of cells with SIX1 overexpression (n = 3) (E). MTT assays of BCPAP cells with ectopic expression of SIX1 (n = 5) (F). Growth curve assays of BCPAP cells with ectopic expression of SIX1 (n = 5) (G). Representative images of colony formation assays of BCPAP cells with ectopic expression of SIX1 and quantitative results (n = 3) (H). The representative images of migration and invasion assay in BCPAP cells with SIX1 overexpression and quantitative analysis (n = 3) (I). *p < 0.05.