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. 2019 Dec 20;9:1450. doi: 10.3389/fonc.2019.01450

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Copyright © 2019 Kong, Li, Liu, Cui, Wang, Zhang, Tang, Du, Liu, Wu and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown SIX1 decreased the proportion of cells in S phase and inhibited proliferation, migration, and invasion in TPC-1 cell lines. Representative images of cell cycle plot (A) and quantitative results (n = 3) (B). Quantitative results of EdU staining of cells with SIX1 knockdown (n = 3) (C). MTT assays of TPC-1 cells after silencing SIX1 (n = 5) (D). Growth curve assays of TPC-1 cells after silencing SIX1 (n = 5) (E). Representative images of colony formation assays of TPC-1 cells after silencing SIX1 and quantitative results (n = 3) (F). The representative images of migration and invasion assay in TPC-1 cells after silencing SIX1 and quantitative analysis (n = 3) (G). ^*p < 0.05.