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. 2019 Dec 20;9:1450. doi: 10.3389/fonc.2019.01450

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Copyright © 2019 Kong, Li, Liu, Cui, Wang, Zhang, Tang, Du, Liu, Wu and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SIX1 attenuated H₂O₂-induced apoptosis in PTC cell lines. PTC cell lines were incubated for 24 h, and then exposed to 200 μM H₂O₂ for 24 h. Annexin V-FITC/PI assay was used to evaluate the apoptosis. Representative image of apoptosis assay and quantitative analysis (n = 3) (A). The protein expression of caspase 3, cleaved PARP1 and cleaved caspase 9 in PTC cell lines was detected by Western blotting (B). ^*p < 0.05.