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. 2019 Dec 27;12:298. doi: 10.1186/s13068-019-1638-x

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© The Author(s) 2019

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Growth curves of S. cerevisiae BY4742 and Atg22Δ (a), and cell survival of both strains after acetic acid treatment (b). The strains were cultured in SC media (pH 3.0) with acetic acid (Ac) or without 150 mM Ac (CK), and cultures at different times were assayed by optical density at 600 nm (OD₆₀₀) (a). An enlarged image of growth curve during acetic acid treatment is illustrated in the insert. Viability of yeast cells treated with different concentration of acetic acid for 2 h were determined by assaying colony-forming units (CFU) after 3 days cultivation on YPD agar plates (b). The data represent mean ± SD (n = 3; *P < 0.05)