Table 2.

Tumorigenicity and metastatic ability of U-2OS cells after the depletion of ROCK2

Cells	Tumor			Lung metastases
	Incidence	Latency (mean days ± SEM)	Volume at 9 weeks (mean cm3 ± SEM)	Incidence	Median number	Individual values
U-2/SCR pool	6/6	23.2 ± 1.2	1.635 ± 0.051	6/6	> 200	> 200, > 200, > 200, > 200, > 200, > 200
U-2/sh ROCK2 #78	6/6	37 ± 2.9	0.958 ± 0.233*	0/6†	0‡	0, 0, 0, 0, 0, 0
U-2/sh ROCK2 #46	0/6	NA	0**	0/6†	0‡	0, 0, 0, 0, 0, 0

†p = 0.01, Fisher’s exact test, vs U-2/SCR pool

^*p < 0.05, ^**p <0.0001, Student’s t test, vs. U-2/SCR pool

‡p < 0.01, Wilcoxon’s rank sum test, vs. U-2/SCR pool