Figure 2.

Maternal protein restriction leads to increased markers of hepatic oxidative stress at 4 months of age. (A) Representative Western immunoblots illustrating expression of 4-hydroxynonenal, a marker of lipid peroxidation, superoxide dismutase (SOD)1 and SOD2 in LP1, LP2 and LP3 offspring relative to control diet-fed offspring. Protein abundances of (B) 4HNE, (C) SOD1, (D) SOD2, and (E) catalase in each group of MPR offspring were each compared against control offspring. 4HNE abundance was expressed as means normalized to total protein abundance ± s.e.m. (n = 7–8/group), while SOD1, SOD2 and catalase abundance was normalized to β-Actin ± s.e.m. (n = 7–8/group). All protein abundances were analyzed using a two-tailed unpaired Student’s t-test. *Significant difference (P < 0.05), **significant difference (P < 0.01), ***significant difference (P < 0.001).