Figure 5.

Image analysis with normal modes. (a) and (b) Two sections of the dialog box, regarding input (panel a) and alignment (panel b). (c) An example of visualization of image analysis results in three dimensions (amplitudes along three normal modes). The image analysis task is parallelized using MPI protocol and the number of MPI cores (parallel processes that do not necessarily share the same memory) can be specified in the MPI field (panel a). In panel b, the default choice for the “Rigid‐body alignment method” is “wavelets & splines” (the alternative method is “projection matching”) and the elastic alignment is performed by Powell's trust region method. In panel c, each point corresponds to a particle image with assigned orientations, translations, and normal‐mode displacement amplitudes with respect to the reference model (close points correspond to similar conformations and vice versa)