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. 2019 Dec 26;20:1019. doi: 10.1186/s12864-019-6391-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Volcano plots of DEGs and overlaps in DEGs in response to infection. a Volcano plots showing the distribution of normalized read counts as a function of Log₂(fold-change) for DEGs in each of the four comparisons. Negative and positive Log₂(fold-change) describe down-regulated and up-regulated genes, respectively, relative to the reference treatment, which is always the denominator of the title (e.g for S0/R0, R0 is the reference). b and c Overlap in number of genes more highly expressed at days 0 and 4 (b) in susceptible compared to resistant fish and c in resistant compared to susceptible fish. d and e Overlap in number of genes between the response to infection of resistant (R4/R0) and susceptible (S4/S0) fish divided to (d) up-regulated and (e) down-regulated DEGs