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. 2019 Dec 26;20:1019. doi: 10.1186/s12864-019-6391-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Relative expression of representative genes at further time points. Expression levels in susceptible (red bars) and resistant (green bars) fish at different days were measured using RT-qPCR, normalized to those of ef1a and the fold changes were calculated relative to the mean of one resistant family at day 0. Each panel refers to a different gene that was initially found to be differentially expressed between day 0 and 4 in susceptible or resistant fish in the RNA-Seq analysis. a and b interferon induced genes. Both vip2 (a) and mxe (b) were up-regulated only in susceptible fish between day 0 and 4 based on the RNA-Seq data. c to e chemokine genes. Comparing the RNA-Seq data between days 0 and 4 identified ccl19a1.1 (c) was up-regulated only in susceptible fish, while cxcl8b1.2 (d) and cxcl8b1.3 (e) were up-regulated only in resistant fish. None of the within-day comparisons between resistant and susceptible fish were statistically significant. In all panels, changes in expression that were significantly different from day 0 within the same fish type are marked by asterisks (*)