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. Author manuscript; available in PMC: 2020 Dec 19.
Published in final edited form as: Mol Cell. 2019 Oct 24;76(6):938–952.e5. doi: 10.1016/j.molcel.2019.09.025

Figure 6. Structures of Nme1Cas9-sgRNA in complex with AcrIIC3 or with both AcrIIC3 and dsDNA.

Figure 6.

A. AcrIIC3 binds to Nme1Cas9 in the apo, sgRNA-bound, and sgRNA-dsDNA-bound states.

B. Architecture of the Nme1Cas9-sgRNA in complex with AcrIIC3, showing that two AcrIIC3 monomers fasten two Nme1Cas9-sgRNA complexes together.

C. Architecture of the Nme1Cas9-sgRNA in complex with AcrIIC3 and dsDNA, showing that AcrIIC3 does not fully prevent target DNA binding.

See also Figure S6 and Table S3.