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. 2019 Dec 20;10:2992. doi: 10.3389/fimmu.2019.02992

Figure 3.

Figure 3

Effect of the combination of Methotrexate or Methylprednisolone and biotherapy on pro-inflammatory cytokine production. Healthy activated-PBMC were pre-incubated or not with Methotrexate alone (0.01 μg/ml), biotherapy alone (10 μg/ml), or with the combination of both treatments. Then, PBMC were co-cultured with RA synoviocytes at a ratio 5:1 for 48 h. Infliximab, Tocilizumab, Abatacept and Rituximab were tested. After 48 h, the production of IL-17, IL-6, IL-1β, and IFN-γ in cell supernatants was measured by ELISA. Data from our previous study were used for methylprednisolone (MP) results (10). *, #p ≤ 0.05. *compares with control and # compares combination MTX/biotherapy or MP/biotherapy vs. MTX alone or MP alone, respectively. Results are represented as mean ± SEM, n = 6 experiments from five RA patients for results with MTX and n = 11 experiments from six RA patients for results with MP.