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. 2019 Dec 20;7:350. doi: 10.3389/fcell.2019.00350

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Copyright © 2019 Xu, Wang, Li, Heindl, He, Yu, Yang, Ge, Ruan, Jia and Fan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LINC-PINT inhibited melanoma progression in vitro. (A) The LINC-PINT overexpression plasmid was stably transfected into A375, Mum2B and CRMM1 cells, and the plasmid also contained the EGFP tag. Scale bars: 100 μm. Real-time PCR results showed significantly higher expression of LINC-PINT in A375-PINT oe, M2B-PINT oe and CM1-PINT oe cells. ^∗∗P < 0.01. (B) CCK8 assay was performed to assess cell proliferation in the Mock and LINC-PINT overexpression melanoma cells. ^∗∗p < 0.01. (C) Colony count statistics demonstrated tumor formation ability. The colony count statistics showed a significant reduction in the numbers of LINC-PINT overexpressed A375, Mum2B and CRMM1 cells. The colony numbers were determined from three independent soft agar plates. ^∗∗P < 0.01. (D) Cell cycle analysis by flow cytometry was performed to determine the percentage of cells in different cell cycle phases. The percentage of cells in G0/G1 phase increased after LINC-PINT overexpression in A375, Mum2B and CRMM1 cells. All histograms showed the percentage (%) of cell populations from one independent experimental group. (E) The migration and invasion abilities of LINC-PINT overexpressed melanoma cells were detected by transwell assay.