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. 2019 Dec 3;130(1):345–358. doi: 10.1172/JCI130391

Figure 5. Mice treated with GBP recover forelimb function after cervical SCI.

Figure 5

(A) Experimental scheme. (B) Recovery of forelimb skilled locomotor function was measured using the horizontal ladder rung-walking test. Mean and SEM (mixed model with repeated measures using compound symmetry covariance structure and controlled on baseline values ***P < 0.001; vehicle n = 15 and GBP n = 15 mice). (C) Replication of the study shown in B. Mean and SEM (mixed model with repeated measures using compound symmetry covariance structure and controlled on baseline values, **P < 0.01; vehicle n = 10 and GBP n = 10 mice). (D) Photographs showing forelimb footprints used for intermediary toe spread measurements. Green circles indicate normal toe spread. (E) Quantification of D. Box and whiskers (minimum to maximum) (2-way ANOVA followed by Tukey’s post test **P < 0.001; NS, not significant; vehicle n = 10 and GBP n = 9 mice). (F) Coronal sections of the brain were stained using NeuN and mCherry antibodies. Scale bar: 200 μm. Transient activation of hM4Di in corticospinal neurons abrogates recovery of (G) forelimb skilled walking and (H) forelimb symmetry (cylinder test) in rearing after C5 SCI in mice receiving GBP. Scatter plot (2-way ANOVA followed by Bonferroni’s post test ***P < 0.001; NS, not significant; vehicle n = 14 and GBP n = 11 mice).