Figure 3. Single-cell RNA sequencing confirms the existence of 2 distinct brown adipocyte subpopulations.

(A) t-distributed stochastic neighbor embedding (tSNE) plot of 3602 primary brown adipocytes isolated from 10-week-old WT male mice. Clustering was generated using k-means = 4. These data are from a single experiment. (B–F) Transcript counts represent log₂ of gene expression. Each dot corresponds to one single cell, colored according to cell cluster. (B) Violin plots showing the distribution of normalized expression values of adiponectin (Adipoq) and Ucp1 across cells that belong to the 5 adipocyte clusters. (C–F) Distribution of the expression of Adipoq and Ucp1 (C), Cidea, Elovl6, Cox7a1, and Ndufa12 (D), Lipe (Hsl) and Pnpla2 (Atgl) (E), and Fabp4 and Cd36 (F) within tSNE plot.