Figure 10. VIT-2763 decreased apoptosis and extended the life span of RBCs in Hbb^th3/+ mice.

(A) VIT-2763 lowered PS exposure on peripheral RBCs, as detected by decrease in intensity of annexin V staining. n = 9–10. (B) VIT-2763 reduced the expression of liver Hmox1, as detected by qPCR. n = 10–12. (A and B) Individual values and mean ± SD are shown. Statistical analysis was performed by comparing all treatment groups to the Hbb^th3/+ vehicle group using 1-way ANOVA with Dunnett’s multiple comparison test. (C) VIT-2763 (60 mg/kg for 7 weeks) extended the life span of RBCs in Hbb^th3/+ mice. Biotin labeling was performed after 21 days of dosing with VIT-2763. Shown is the percentage of biotinylated Ter119⁺ cells normalized to the percentage of labeled cells at day 1 after biotin injection. n = 4–10. Mean ± SD values are shown. Statistical analysis was performed using repeated measures 2-way ANOVA with Dunnett’s multiple comparison test to compare all treatment groups to the Hbb^th3/+ vehicle group over time. *P < 0.05; **P < 0.01; ***P < 0.001.