Figure 11. VIT-2763 reduced hypoxia response in RBCs, excessive serum EPO, and Erfe expression in spleens of Hbb^th3/+ mice without effect on liver Hamp.

(A) Percentage of hypoxic RBCs (left, dot plots) and MFI of the hypoxia probe (right, Hypoxia probe⁺ RBCs) in peripheral blood of Hbb^th3/+ mice or WT mice, as detected by flow cytometry analysis. n = 10–13 mice per group. Representative dot plots showing 1 out of 2 independent experiments. (B) Serum EPO was measured by ELISA. n = 10–13. (C) Spleen Erfe (Fam132) (n = 4–13 mice per group) and liver Hamp (D) gene expression were measured by qPCR (n = 10-11 mice). (A–D) Individual values and mean ± SD are shown. Statistical analysis was performed by comparing all treatment groups to the Hbb^th3/+ vehicle group using 1-way ANOVA with Dunnett’s multiple comparison test. *P < 0.05; ***P < 0.001.