Figure 12. Effects of VIT-2763 on myeloid precursors in spleens of Hbb^th3/+ mice.

(A) Gating strategy used to identify myeloid cell populations from the spleen of WT and Hbb^th3/+ mice: (i) mature neutrophils (Ly6G^hiLy6C^int),(ii) immature myeloid cells (Ly6G^intLy6C^int), (iii) resident monocytes (Ly6G^negLy6C^int), and (iv) inflammatory monocytes (Ly6G^negLy6C^hi). Representative dot plots from 1 out of 3 independent experiments are shown. (B) Quantification of percentages of mature neutrophils, immature myeloid cells, resident monocytes, and inflammatory monocytes in CD11b⁺ spleen cells. Individual values and mean ± SD are shown. n = 8–13 mice per group. Statistical analysis was performed by comparing all treatment groups to the Hbb^th3/+ vehicle group using 1-way ANOVA with Dunnett’s multiple comparison test. ***P < 0.001.