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. 2019 Dec 9;130(1):491–506. doi: 10.1172/JCI129382

Figure 7. VIT-2763 treatment improved the ineffective erythropoiesis in BM and spleen of Hbbth3/+ mice.

Figure 7

Gating strategy used to identify erythroid progenitors in BM (A) or spleen (D) by flow cytometry. Representative dot plots from 1 out of 4 independent experiments showing vehicle- or VIT-2763-treated Hbbth3/+ and WT mice. VIT-2763 decreased the frequency of polychromatic erythroblasts (population in gate 3) in BM (B) and spleen (E). VIT-2763 treatment reduced the percentages of BM (C) and spleen (F) ROS-positive mature erythrocytes. (B and E) Black symbols show polychromatic erythroblasts, and gray symbols show mature erythrocytes. (B, C, E and F) x axis labels: 1, vehicle; 2, VIT-2763 (30 mg/kg); 3, VIT-2763 (100 mg/kg). Individual values and mean ± SD are shown. Statistical analysis was performed by comparing all treatment groups to the Hbbth3/+ vehicle group using 1-way ANOVA with Dunnett’s multiple comparison test. n = 10–12 mice. **P < 0.01; ***P < 0.001.